|
KMID : 1094720190240020282
|
|
Biotechnology and Bioprocess Engineering
2019 Volume.24 No. 2 p.282 ~ p.287
|
|
|
Anti-cariogenic Characteristics of Rubusoside
|
|
Kim Jee-Soo
Nguyen Thi Thanh Hanh
Jin Ju-Hui
Septiana Iis
Son Gyu-Min
Lee Gang-Hee
Jung You-Jin
Qureshi Dilshad
Mok Il-Kyoon
Pal Kunal
Yang Soo-Yeon
Kim Seong-Bo
Kim Do-Man
|
|
|
Abstract
|
|
|
|
Streptococcus mutans plays an important role in the development of dental caries in humans by synthesizing adhesive insoluble glucans from sucrose by mutansucrase activity. To explore the anti-cariogenic characteristics of rubusoside (Ru), a natural sweetener component in Rubus suavissimus S. Lee (Rosaceae), we investigated the inhibitory effect of Ru against the activity of mutansucrase and the growth of Streptococcus mutans. Ru (50 mM) showed 97% inhibitory activity against 0.1 U/mL mutansucrase of S. mutans with 500 mM sucrose. It showed competitive inhibition with a Ki value of 1.1 ± 0.2 mM and IC50 of 2.3 mM. Its inhibition activity was due to hydrophobic and hydrogen bonding interactions based on molecular docking analysis. Ru inhibited the growth of S. mutans as a bacteriostatic agent, with MIC and MBC values of 6 mM and 8 mM, respectively. In addition, Ru showed synergistic anti-bacterial activity when it was combined with curcumin. Therefore, Ru is a natural anti-cariogenic agent with anti-mutansucrase activity and antimicrobial activity against S. mutans.
|
|
|
KEYWORD
|
|
|
Anti-cariogenicity, mutansucrase, natural sweetener, rubusoside, Streptococcus mutans
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
 
|